6XUP
Archaeoglobus fulgidus Argonaute protein with DNA oligoduplex 5'-pATCGTGGCCACGAT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97630 |
Spacegroup name | P 1 |
Unit cell lengths | 51.798, 60.872, 101.718 |
Unit cell angles | 76.56, 75.59, 79.39 |
Refinement procedure
Resolution | 40.716 - 1.900 |
R-factor | 0.1856 |
Rwork | 0.181 |
R-free | 0.22430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdbid 2w42 |
Data reduction software | XDS |
Data scaling software | pointless (1.10.26) |
Phasing software | MOLREP (11.6.04) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.500 | 41.500 | 1.940 |
High resolution limit [Å] | 1.900 | 9.870 | 1.900 |
Rmerge | 0.053 | 0.030 | 0.955 |
Rmeas | 0.062 | 0.036 | 1.106 |
Rpim | 0.032 | 0.019 | 0.557 |
Number of reflections | 91396 | 572 | 4556 |
<I/σ(I)> | 11.2 | ||
Completeness [%] | 91.6 | 87.9 | 91.2 |
Redundancy | 3.8 | 3.5 | 3.9 |
CC(1/2) | 0.999 | 0.997 | 0.629 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 280 | Na-Cacodylate pH 5.5 0.05 M, KCl 0.12 M, MgCl2 0.01 M, PEG3350 7%, DTT_0.005 M, glycerol 5% (w/v) |