6XU2

Human karyopherin RanBP5 (isoform-3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.99987
Spacegroup name	P 21 21 21
Unit cell lengths	97.097, 126.248, 149.419
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	96.430 - 2.834
R-factor	0.2079
Rwork	0.206
R-free	0.24430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xte
RMSD bond length	0.009
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	3.060
High resolution limit [Å]	2.830	2.830
Rmerge	0.074	1.124
Rpim	0.035	0.503
Number of reflections	35901	1796
<I/σ(I)>	11.2	1.4
Completeness [%]	81.0	20.3
Redundancy	5.6	5.7
CC(1/2)	0.996	0.625

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20 % PEG 3350, 100 mM sodium di-hydrogen phosphate