6XTE

Human karyopherin RanBP5 (isoform-1)

Summary for 6XTE

• Entry DOI: 10.2210/pdb6xte/pdb
• Related PRD ID: PRD_000180
• Descriptor: Importin-5, Antipain, NICKEL (II) ION, ... (5 entities in total)
• Functional Keywords: human karyopherin, pa-pb1 sub-complex nuclear import, influenza polymerase assembly, host-pathogen interaction, translocase
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 2
• Total formula weight: 124457.04

Authors

Swale, C.,McCarthy, A.A.,Ruigrok, R.W.H.,Crepin, T. (deposition date: 2020-01-16, release date: 2020-04-22, Last modification date: 2024-10-23)

Primary citation

Swale, C.,Da Costa, B.,Sedano, L.,Garzoni, F.,McCarthy, A.A.,Berger, I.,Bieniossek, C.,Ruigrok, R.W.H.,Delmas, B.,Crepin, T.
X-ray Structure of the Human Karyopherin RanBP5, an Essential Factor for Influenza Polymerase Nuclear Trafficking.
J.Mol.Biol., 432:3353-3359, 2020

PubMed Abstract: Here, we describe the crystal structures of two distinct isoforms of ligand-free human karyopherin RanBP5 and investigate its global propensity to interact with influenza A virus polymerase. Our results confirm the general architecture and mechanism of the IMB3 karyopherin-β subfamily whilst also highlighting differences with the yeast orthologue Kap121p. Moreover, our results provide insight into the structural flexibility of β-importins in the unbound state. Based on docking of a nuclear localisation sequence, point mutations were designed, which suppress influenza PA-PB1 subcomplex binding to RanBP5 in a binary protein complementation assay.

PubMed: 32222384
DOI: 10.1016/j.jmb.2020.03.021

Experimental method

X-RAY DIFFRACTION (2.27 Å)