6XM2
The structure of the 4A11.v7 antibody in complex with human TGFb2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.415, 85.108, 112.732 |
| Unit cell angles | 99.15, 99.51, 97.32 |
Refinement procedure
| Resolution | 82.970 - 1.910 |
| Rwork | 0.205 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kxz |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.970 | 2.010 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.073 | 0.494 |
| Number of reflections | 161613 | 8036 |
| <I/σ(I)> | 8.5 | 2.5 |
| Completeness [%] | 90.5 | 88.7 |
| Redundancy | 2.8 | 2.9 |
| CC(1/2) | 0.997 | 0.877 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 20% PEG 1000, 0.1 M Tris pH8.5 |
