6XM2

The structure of the 4A11.v7 antibody in complex with human TGFb2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	64.415, 85.108, 112.732
Unit cell angles	99.15, 99.51, 97.32

Refinement procedure

Resolution	82.970 - 1.910
Rwork	0.205
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kxz
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.970	2.010
High resolution limit [Å]	1.910	1.910
Rmerge	0.073	0.494
Number of reflections	161613	8036
<I/σ(I)>	8.5	2.5
Completeness [%]	90.5	88.7
Redundancy	2.8	2.9
CC(1/2)	0.997	0.877

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	20% PEG 1000, 0.1 M Tris pH8.5