6XLV
Crystal structure of leukemia-associated N196K mutant of U2AF65 bound to AdML splice site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.335, 63.048, 76.892 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.450 - 1.400 |
R-factor | 0.143 |
Rwork | 0.141 |
R-free | 0.16860 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5ev3 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.209 |
Data reduction software | XDS (Mar 15, 2019) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHENIX (1.17.1) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.450 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.055 | 0.677 |
Rpim | 0.024 | 0.381 |
Number of reflections | 76426 | 1439 |
<I/σ(I)> | 13.1 | 1.3 |
Completeness [%] | 96.0 | 70.7 |
Redundancy | 6.2 | 3.8 |
CC(1/2) | 0.998 | 0.703 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG 3350, 3.4 M sodium malonate pH 7, sucrose, LDAO |