6XKI
Crystal structure of eIF4A-I in complex with RNA bound to des-MePateA, a pateamine A analog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.987 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 66.785, 99.942, 153.663 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.580 - 2.870 |
R-factor | 0.2238 |
Rwork | 0.222 |
R-free | 0.26230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zc9 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.470 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18_3861) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.870 | 2.870 |
Number of reflections | 11588 | 453 |
<I/σ(I)> | 12.25 | |
Completeness [%] | 96.6 | |
Redundancy | 11.4 | |
CC(1/2) | 0.984 | 0.811 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 0.1 M MES pH 6.7, 1.9 M Ammonium Sulfate |