6XID
PCSK9(deltaCRD) in complex with cyclic peptide 51
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-08 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 70.827, 70.827, 152.952 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 61.340 - 1.482 |
R-factor | 0.1877 |
Rwork | 0.187 |
R-free | 0.20490 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4nmx |
RMSD bond length | 0.008 |
RMSD bond angle | 0.960 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | MOLREP |
Refinement software | BUSTER (2.11.7 (6-FEB-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.340 | 1.547 |
High resolution limit [Å] | 1.482 | 1.482 |
Number of reflections | 74593 | 3427 |
<I/σ(I)> | 13.2 | |
Completeness [%] | 92.3 | |
Redundancy | 9.8 | |
CC(1/2) | 0.999 | 0.559 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 20% PEG3350, 200mM CaCl2, 100mM MES pH 6 |