6XHY

Crystal structure of the Thermus thermophilus 70S ribosome in complex with telithromycin, mRNA, aminoacylated A- and P-site tRNAs, and deacylated E-site tRNA at 2.60A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-04
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	209.400, 448.120, 618.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	224.060 - 2.600
R-factor	0.2193
Rwork	0.217
R-free	0.26560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4p
RMSD bond length	0.004
RMSD bond angle	0.850
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	224.060	224.060	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.177	0.040	1.513
Rmeas	0.209	0.047	1.785
Total number of observations	6040953
Number of reflections	1730665	20036	126907
<I/σ(I)>	6.2	20.52	0.83
Completeness [%]	98.4	96.5	98
Redundancy	3.491	3.565	3.423
CC(1/2)	0.987	0.997	0.131

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME