6X6E
Glucocorticoid Receptor DNA binding domain in complex with methylated precursor for a modern recognition element (methylated pre-GBS)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-21 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 130.385, 39.089, 97.371 |
Unit cell angles | 90.00, 118.62, 90.00 |
Refinement procedure
Resolution | 36.990 - 2.000 |
R-factor | 0.1832 |
Rwork | 0.181 |
R-free | 0.21220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fyl |
RMSD bond length | 0.007 |
RMSD bond angle | 0.880 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.991 | 2.072 |
High resolution limit [Å] | 2.000 | 2.001 |
Rmeas | 0.194 | |
Rpim | 0.076 | |
Number of reflections | 29271 | 2652 |
<I/σ(I)> | 16.5 | |
Completeness [%] | 98.7 | 90.22 |
Redundancy | 6.7 | 4 |
CC(1/2) | 0.692 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298.15 | 50 mM sodium cacodylate pH 6.5, 80 mM calcium chloride, 1 % glycerol, and 7 % PEG 400 |