6X5H
Human Alpha-1,6-fucosyltransferase (FUT8) bound to GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 65 |
| Unit cell lengths | 173.490, 173.490, 207.420 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 86.745 - 2.250 |
| R-factor | 0.1721 |
| Rwork | 0.171 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2de0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.918 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.750 | 2.310 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmeas | 0.158 | 1.100 |
| Number of reflections | 165616 | 11970 |
| <I/σ(I)> | 10.44 | |
| Completeness [%] | 99.2 | 97.1 |
| Redundancy | 5.1 | 4.1 |
| CC(1/2) | 0.995 | 0.556 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.6 M Ammonium sulfate, 0.6 M L-proline, 100 mM Hepes pH 7.5 |
