6WZW

Ash1L SET domain in complex with AS-85

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-23
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9786
Spacegroup name	C 1 2 1
Unit cell lengths	76.277, 31.998, 91.160
Unit cell angles	90.00, 102.34, 90.00

Refinement procedure

Resolution	37.330 - 1.690
R-factor	0.1674
Rwork	0.164
R-free	0.20630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ynm
RMSD bond length	0.007
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.330	1.720
High resolution limit [Å]	1.690	1.690
Rmerge	0.105	0.483
Number of reflections	24518	2374
<I/σ(I)>	14.944
Completeness [%]	99.6
Redundancy	3.7
CC(1/2)		0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	290	0.1 M HEPES pH 7.5, 30% PEG3350