6WXJ
CSF1R signaling is a regulator of pathogenesis in progressive MS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X17B1 |
| Synchrotron site | NSLS |
| Beamline | X17B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-14 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.9201 |
| Spacegroup name | H 3 |
| Unit cell lengths | 80.520, 80.520, 144.060 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.020 - 2.620 |
| R-factor | 0.1847 |
| Rwork | 0.181 |
| R-free | 0.27230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dpk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.921 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.020 | 2.690 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.100 | 1.100 |
| Number of reflections | 10436 | 10436 |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 99.5 | |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | 0.1 M MES, pH 6.5, 21.3% polyethylene glycol 3350 and 0.25 M ammonium sulfate |
