6WWH
KIF14[391-772] dimer two-heads-bound state - AMP-PNP in complex with a microtubule
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.8 |
| Software | UCSF Chimera,Coot,SPIDER,RELION,Leginon,RosettaEM,PHENIX,MODELLER |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
