6WWA

Crystal structure of human SHLD2-SHLD3-REV7 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-16
Detector	PSI PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	I 4 3 2
Unit cell lengths	331.842, 331.842, 331.842
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.660 - 3.800
R-factor	0.2403
Rwork	0.239
R-free	0.26630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3abd
RMSD bond length	0.003
RMSD bond angle	0.697
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.936
High resolution limit [Å]	3.800	3.800
Rmerge	0.403	4.971
Rmeas	0.409	5.040
Rpim	0.068	0.832
Number of reflections	30856	3027
<I/σ(I)>	10.3	0.86
Completeness [%]	99.7
Redundancy	35.8
CC(1/2)	0.999	0.472

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.1 M Na acetate pH 5.6, 1.5 M Na formate