6WT7
Structure of a metazoan TIR-STING receptor from C. gigas in complex with 2',3'-cGAMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-11-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 108.126, 108.126, 71.569 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.820 - 2.900 |
| R-factor | 0.2332 |
| Rwork | 0.231 |
| R-free | 0.25230 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.437 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.820 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rpim | 0.034 | 0.408 |
| Number of reflections | 10981 | 1763 |
| <I/σ(I)> | 12.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12 | |
| CC(1/2) | 0.997 | 0.881 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.2 M ammonium citrate pH 7.0, 20% PEG-3350 |
