6WPH

Structure of HIV-1 Reverse Transcriptase (RT) in complex with dsDNA and (-)-FTC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	C 2 2 21
Unit cell lengths	165.980, 168.054, 101.916
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.520 - 2.720
R-factor	0.1999
Rwork	0.197
R-free	0.25660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6or7
RMSD bond length	0.007
RMSD bond angle	0.942
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.880
High resolution limit [Å]	2.710	2.710
Number of reflections	38359	5620
<I/σ(I)>	20.33	2.68
Completeness [%]	98.4	90.9
Redundancy	25.3	24.1
CC(1/2)	0.999	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	6-10% (w/v) PEG 8000, 15 mM magnesium sulfate, and 50 mM MES adjusted at pH 6.0