6WOL

Next generation monomeric IgG4 Fc bound to neonatal Fc receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-22
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.97946
Spacegroup name	C 2 2 21
Unit cell lengths	68.284, 122.598, 178.456
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.100 - 2.490
R-factor	0.2353
Rwork	0.234
R-free	0.26950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hvw
RMSD bond length	0.005
RMSD bond angle	1.425
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.100	2.600
High resolution limit [Å]	2.490	2.490
Rmerge	0.203	0.847
Rpim	0.086	0.374
Number of reflections	26347	2798
<I/σ(I)>	4.9	1.4
Completeness [%]	99.3
Redundancy	6.4
CC(1/2)	0.984	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	295	0.2 M magnesium chloride hexahydrate, 1 M sodium iodide, 0.1 M MES, pH 6 and 20% PEG 6000 at a protein concentration of 6.35 mg/mL