6WNX

FBXW11-SKP1 in complex with a pSer33/pSer37 Beta-Catenin peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00004
Spacegroup name	P 1 21 1
Unit cell lengths	119.732, 81.866, 133.198
Unit cell angles	90.00, 92.92, 90.00

Refinement procedure

Resolution	133.020 - 2.500
R-factor	0.2081
Rwork	0.206
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p22
RMSD bond length	0.009
RMSD bond angle	1.545
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	133.020	2.650
High resolution limit [Å]	2.500	2.500
Rmerge	0.223	1.478
Rmeas	0.243	1.614
Rpim	0.095	0.637
Number of reflections	78568	4511
<I/σ(I)>	5.7
Completeness [%]	98.9	99.8
Redundancy	6.4	6.3
CC(1/2)	0.984	0.289

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.12M Ethylene Glycol, 0.1M Morpheus Buffer 1 pH 6.5, 30.0% GOL_P4K