6WNA

Next generation monomeric IgG4 Fc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-22
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.1950
Spacegroup name	C 2 2 21
Unit cell lengths	68.095, 122.096, 179.446
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 2.400
R-factor	0.2154
Rwork	0.214
R-free	0.26270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hvw 4n0u
RMSD bond length	0.007
RMSD bond angle	1.841
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.149	1.261
Rpim	0.061	0.519
Number of reflections	29521	3072
<I/σ(I)>	8	1.6
Completeness [%]	99.5	99.5
Redundancy	6.7	6.7
CC(1/2)	0.996	0.631

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	0.2 M magnesium chloride hexahydrate, 30% 1,5-diaminopentane dihydrochloride, 0.1 M MES, pH 6 and 20% PEG 6000 at a protein concentration of 6 mg/mL