6WMO

Human poly-N-acetyl-lactosamine synthase structure demonstrates a modular assembly of catalytic subsites for GT-A glycosyltransferases

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-21
Detector	RAYONIX MX300-HS
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	48.190, 109.280, 147.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.848 - 1.850
R-factor	0.1842
Rwork	0.183
R-free	0.21150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wmm
RMSD bond length	0.010
RMSD bond angle	0.928
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.848	37.848	1.900
High resolution limit [Å]	1.850	8.270	1.850
Rmerge	0.061
Rmeas	0.063	0.027
Total number of observations	947780
Number of reflections	67358	860	4731
<I/σ(I)>	24.4	82.81	1.01
Completeness [%]	99.7	98.7	96.4
Redundancy	14.071	12.285	9.899
CC(1/2)	1.000	1.000	0.449

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	24% PEG3350, 6% Ethylene glycol, 100 mM Hepes pH 8.5