6WMO
Human poly-N-acetyl-lactosamine synthase structure demonstrates a modular assembly of catalytic subsites for GT-A glycosyltransferases
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-21 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.190, 109.280, 147.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.848 - 1.850 |
R-factor | 0.1842 |
Rwork | 0.183 |
R-free | 0.21150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6wmm |
RMSD bond length | 0.010 |
RMSD bond angle | 0.928 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.848 | 37.848 | 1.900 |
High resolution limit [Å] | 1.850 | 8.270 | 1.850 |
Rmerge | 0.061 | ||
Rmeas | 0.063 | 0.027 | |
Total number of observations | 947780 | ||
Number of reflections | 67358 | 860 | 4731 |
<I/σ(I)> | 24.4 | 82.81 | 1.01 |
Completeness [%] | 99.7 | 98.7 | 96.4 |
Redundancy | 14.071 | 12.285 | 9.899 |
CC(1/2) | 1.000 | 1.000 | 0.449 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 24% PEG3350, 6% Ethylene glycol, 100 mM Hepes pH 8.5 |