6WMO

Human poly-N-acetyl-lactosamine synthase structure demonstrates a modular assembly of catalytic subsites for GT-A glycosyltransferases

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2	polymer	364	42220.2	2	UniProt (Q9NY97) Pfam (PF01762)	Homo sapiens (Human)	Beta-1,3-N-acetylglucosaminyltransferase 1,Beta3Gn-T1,3-galactosyltransferase 7,b3Gal-T7,Beta-3-Gx-T7,UDP-Gal:beta-GlcNAc beta-1,UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2,Beta3Gn-T2,UDP-galactose:beta-N-acetylglucosamine beta-1
2	C, E (C, E)	beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-beta-D-galactopyranose	branched		545.5	2	In PDB GlyTouCan (G06580KR)
3	D, F (D, F)	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	2	In PDB GlyTouCan (G81315DD)
4	G, L (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	H, M (A, B)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	2	Chemie (UDP)
6	I, J, N (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
7	K, O, P, R, S (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
8	Q (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
9	T, U (A, B)	water	water		18.0	230	Chemie (HOH)

Sequence modifications

A, B: 35 - 397 (UniProt: Q9NY97)

PDB	External Database	Details
Gly 34	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	84440.4
Branched	Number of molecules	4
	Total formula weight	2588.3
Non-Polymers*	Number of molecules	13
	Total formula weight	1923.0
All*	Total formula weight	88951.7

*Water molecules are not included.