6WDZ

Porcine circovirus 2 Rep domain complexed with a single-stranded DNA 10-mer comprising the cleavage site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	.97910
Spacegroup name	P 64
Unit cell lengths	99.527, 99.527, 73.695
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.100 - 2.030
R-factor	0.2317
Rwork	0.229
R-free	0.28020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xor + modeled 10-mer
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.100	2.103
High resolution limit [Å]	2.030	2.030
Rmerge	0.067	0.362
Rmeas	0.082	0.441
Rpim	0.046	0.247
Number of reflections	26261	2625
<I/σ(I)>	12.49	3.63
Completeness [%]	97.5	98.17
Redundancy	2.9
CC(1/2)	0.996	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1M Ammonium acetate, 25% Polyethylene glycol 3,350, 0.1M Bis-Tris pH 7 soaked in 25% glycerol for cryoprotectant