6WC5

Crystal Structure of a Ternary MEF2B/NKX2-5/myocardin enhancer DNA Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-02-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.032
Spacegroup name	P 21 21 21
Unit cell lengths	66.040, 93.240, 136.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.990 - 2.900
R-factor	0.2074
Rwork	0.205
R-free	0.25090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n6j
RMSD bond length	0.004
RMSD bond angle	1.171
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0232)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.000	3.004
High resolution limit [Å]	2.900	2.900
Number of reflections	18336	1904
<I/σ(I)>	11.32
Completeness [%]	94.3
Redundancy	4.1
CC(1/2)	0.997	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	100 mM HEPES pH 7.0, 18% PEG 2000