6W8V

Crystal structure of mouse DNMT1 in complex with ACG DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.85
Spacegroup name	P 1 21 1
Unit cell lengths	89.240, 152.149, 96.049
Unit cell angles	90.00, 94.29, 90.00

Refinement procedure

Resolution	48.471 - 3.120
R-factor	0.2471
Rwork	0.246
R-free	0.27810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4da4
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.210
High resolution limit [Å]	3.100	6.670	3.100
Rmerge	0.393	0.101	2.063
Rmeas	0.426	0.110	2.267
Rpim	0.164	0.043	0.925
Total number of observations	301241
Number of reflections	45209	4612	4523
<I/σ(I)>	1.6
Completeness [%]	100.0	100	100
Redundancy	6.7	6.6	5.9
CC(1/2)		0.996	0.307

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Sodium Citrate pH 4.8, 10 mM ZnCl2