6W4L
The crystal structure of a single chain H2B-H2A histone chimera from Xenopus laevis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-12-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97931 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 61.793, 44.679, 66.450 |
| Unit cell angles | 90.00, 117.27, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.310 |
| R-factor | 0.1712 |
| Rwork | 0.170 |
| R-free | 0.20630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kx5 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.541 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 26.710 | 26.710 | 1.390 |
| High resolution limit [Å] | 1.310 | 4.160 | 1.310 |
| Rmerge | 0.058 | 0.022 | 0.892 |
| Rmeas | 0.069 | 0.026 | 1.052 |
| Rpim | 0.036 | 0.014 | 0.550 |
| Total number of observations | 3963 | 19786 | |
| Number of reflections | 38073 | 1134 | 5487 |
| <I/σ(I)> | 5.6 | 13.9 | 1.1 |
| Completeness [%] | 99.4 | 89.4 | 99.8 |
| Redundancy | 3.7 | 3.5 | 3.6 |
| CC(1/2) | 0.999 | 0.999 | 0.619 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.9 | 291 | 0.2M sodium thiocyanate, 20% PEG3350 |
