6W3J

Crystal structure of the FAM46C/Plk4/Cep192 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	I 2 2 2
Unit cell lengths	88.033, 161.449, 167.471
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.166 - 4.385
R-factor	0.2683
Rwork	0.263
R-free	0.31390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n7z
RMSD bond length	0.004
RMSD bond angle	1.075
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.480
High resolution limit [Å]	4.380	4.380
Rpim	0.023
Number of reflections	7771	367
<I/σ(I)>	26.9
Completeness [%]	99.1
Redundancy	18.7
CC(1/2)		0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	10	293	0.2 M Tris, pH 10