6W3H
Brain delivery of therapeutic proteins using an Fc fragment blood-brain barrier transport vehicle in mice and monkeys
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-20 |
| Detector | DECTRIS PILATUS3 100K-M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 64 |
| Unit cell lengths | 126.370, 126.370, 113.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.300 - 3.380 |
| R-factor | 0.2415 |
| Rwork | 0.238 |
| R-free | 0.31820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3s7g |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.770 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.300 | 3.440 |
| High resolution limit [Å] | 3.380 | 3.380 |
| Number of reflections | 14541 | 713 |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 99.7 | |
| Redundancy | 9.5 | |
| CC(1/2) | 0.930 | 0.560 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297.15 | 20-25% PEG3350, 0.1M Bis-Tris pH6.5, and 0.2M LiSO4 |
