6W10

Structure of mouse TREX1 with E198K disease-associated mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-11-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	65.579, 85.338, 99.465
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.082 - 1.800
R-factor	0.178623584216
Rwork	0.178
R-free	0.20354
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mxj
RMSD bond length	0.010
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.082	1.840
High resolution limit [Å]	1.800	1.800
Rpim	0.044	0.493
Number of reflections	52490	3071
<I/σ(I)>	8.3	1.3
Completeness [%]	99.9	99.4
Redundancy	13.5	13.1
CC(1/2)	0.996	0.694

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M NH4OAc, 17.5% PEG 3350