6W0O
Amyloid-beta(1-40) fibril derived from Alzheimer's disease cortical tissue
Spectrometer
Experimental method: ELECTRON MICROSCOPY, SOLID-STATE NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Varian | InfinityPlus | 600 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | 3D NCACX |
| 2 | 1 | 1 | 3D NCOCX |
| 3 | 1 | 1 | 3D CANCX |
| 4 | 1 | 1 | 3D CONCX |
| 5 | 1 | 1 | 2D 13C-13C |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 7.4 | 1 | 297 |
Conformers
| Conformers Calculated Total Number | 60 |
| Conformers Submitted Total Number | 10 |
EM specimen
| Vitrification | Yes |
| Instrument | LEICA PLUNGER |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Quantum LS |
| Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 2.77 |
| Number of Particle | 19790 |
| Software | RELION,MATLAB,SerialEM,Gctf,Coot,X-PLOR |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
