6W0M
Human 8-oxoguanine glycosylase crosslinked with oxoG lesion containing DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 91.678, 91.678, 212.106 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.840 - 2.370 |
R-factor | 0.2 |
Rwork | 0.197 |
R-free | 0.26550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ebm |
RMSD bond length | 0.009 |
RMSD bond angle | 1.448 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.840 | 2.500 |
High resolution limit [Å] | 2.370 | 2.370 |
Rmerge | 0.086 | 0.746 |
Number of reflections | 22361 | 3154 |
<I/σ(I)> | 35.2 | |
Completeness [%] | 99.8 | |
Redundancy | 20.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM sodium cacodylate, pH 6.5, 200 mM MgCl2, and 18 % polyethylene glycol 8000. |