6VVU
Anti-Tryptase fab E104.v1 bound to tryptase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2015-01-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 89.573, 168.811, 114.652 |
Unit cell angles | 90.00, 109.97, 90.00 |
Refinement procedure
Resolution | 90.830 - 3.000 |
R-factor | 0.188 |
Rwork | 0.187 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fvd |
RMSD bond length | 0.010 |
RMSD bond angle | 1.190 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.6) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.830 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.860 | |
Rpim | 0.582 | |
Number of reflections | 62323 | 6175 |
<I/σ(I)> | 11.3 | 1.37 |
Completeness [%] | 97.8 | 97.8 |
Redundancy | 3.4 | 3.1 |
CC(1/2) | 0.415 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 292 | 0.1 M Tris pH 8.5, 0.2 M CaCl2, 20% PEG 4000 and 4% pentaerythritol ethoxylate |