6VR5
Complex of HLA-A2, a class I MHC, with a p53 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-08-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.596, 79.656, 85.862 |
| Unit cell angles | 90.00, 102.11, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.380 |
| R-factor | 0.2314 |
| Rwork | 0.229 |
| R-free | 0.27980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d2l |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.856 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.100 | 2.470 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.108 | 0.415 |
| Number of reflections | 37994 | 3748 |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 99.9 | |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 295 | 15% PEG 8000, 0.1 M Tris-HCl, 0.2 M magnesium chloride |
