6VR3

Aminoglycoside N-2'-Acetyltransferase-Ia [AAC(2')-Ia] in complex with acetylated-netilmicin and CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	LIQUID ANODE
Source details	BRUKER METALJET
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-25
Detector	Bruker PHOTON II
Wavelength(s)	1.34
Spacegroup name	P 32 2 1
Unit cell lengths	73.038, 73.038, 145.550
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.490 - 2.000
R-factor	0.1834
Rwork	0.180
R-free	0.22600
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	6vr2
RMSD bond length	0.008
RMSD bond angle	0.866
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	PHENIX ((1.17_3644: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.490	2.071
High resolution limit [Å]	2.000	2.000
Number of reflections	58685	3059
<I/σ(I)>	20.82
Completeness [%]	99.9
Redundancy	2
CC(1/2)	0.999	0.761

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	NaSCN, MPD, Acetyl CoA, Netilmicin