6VO7

Crystal structure of PI3K-alpha Ras Binding Domain (RBD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	C 2 2 21
Unit cell lengths	71.548, 83.914, 63.988
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.090 - 2.310
R-factor	0.2027
Rwork	0.197
R-free	0.25520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xgh
RMSD bond length	0.011
RMSD bond angle	0.950
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.090	2.450
High resolution limit [Å]	2.310	2.310
Rmerge	0.171	0.832
Rmeas	0.189
Rpim	0.079	0.391
Number of reflections	8574	414
<I/σ(I)>	21	1.7
Completeness [%]	98.8	97.4
Redundancy	5.8	5
CC(1/2)	0.994	0.679

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1 M Tris-HCL, 0.7 M Na Citrate, pH 8.5