6VO7
Crystal structure of PI3K-alpha Ras Binding Domain (RBD)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 71.548, 83.914, 63.988 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.090 - 2.310 |
R-factor | 0.2027 |
Rwork | 0.197 |
R-free | 0.25520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xgh |
RMSD bond length | 0.011 |
RMSD bond angle | 0.950 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.090 | 2.450 |
High resolution limit [Å] | 2.310 | 2.310 |
Rmerge | 0.171 | 0.832 |
Rmeas | 0.189 | |
Rpim | 0.079 | 0.391 |
Number of reflections | 8574 | 414 |
<I/σ(I)> | 21 | 1.7 |
Completeness [%] | 98.8 | 97.4 |
Redundancy | 5.8 | 5 |
CC(1/2) | 0.994 | 0.679 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris-HCL, 0.7 M Na Citrate, pH 8.5 |