6VO4
Crystal Structure Analysis of BFL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.550, 43.240, 43.490 |
| Unit cell angles | 90.00, 104.90, 90.00 |
Refinement procedure
| Resolution | 38.220 - 1.740 |
| R-factor | 0.2409 |
| Rwork | 0.239 |
| R-free | 0.28310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5whh |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.220 | 38.230 | 1.770 |
| High resolution limit [Å] | 1.740 | 4.720 | 1.740 |
| Rmeas | 0.063 | 0.042 | 1.990 |
| Rpim | 0.035 | 0.024 | 1.068 |
| Total number of observations | 43051 | 2090 | 2190 |
| Number of reflections | 13579 | 668 | 653 |
| <I/σ(I)> | 11.2 | 30.7 | 0.7 |
| Completeness [%] | 92.3 | 87.3 | 92.9 |
| Redundancy | 3.2 | 3.1 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 3.5M Sodium formate, pH 7.0 |
