6VMJ
Crystal structure of human Complement Factor D with anti-Factor D Fab 20D12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-01-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00003 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 180.843, 180.843, 304.189 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.240 - 2.950 |
R-factor | 0.218 |
Rwork | 0.217 |
R-free | 0.26520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4d9r |
RMSD bond length | 0.010 |
RMSD bond angle | 1.220 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.060 |
High resolution limit [Å] | 2.950 | 6.350 | 2.950 |
Rmerge | 0.135 | 0.042 | 0.633 |
Total number of observations | 767163 | ||
Number of reflections | 106295 | 11214 | 10462 |
<I/σ(I)> | 7 | ||
Completeness [%] | 99.9 | 99.9 | 99.8 |
Redundancy | 7.2 | 7.9 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1 M sodium cacodylate pH 6.5, 1 M sodium citrate |