6VM8

SILv44 T cell receptor bound to HLA-A2 presenting gp100T2M peptide (IMDQVPFSV)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	68.269, 94.445, 143.824
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.170 - 2.410
R-factor	0.1885
Rwork	0.186
R-free	0.22140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.481
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MoRDa
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.149	0.760
Rmeas	0.159	0.854
Rpim	0.053	0.379
Number of reflections	36792	1759
<I/σ(I)>	23.2	2.2
Completeness [%]	99.7	95.8
Redundancy	8.2	4.7
CC(1/2)		0.568

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	20% v/v PEG3350, 236 mM ammonium citrate dibasic