6VJW

Crystal structure of WT hMBD4 complexed with T:G mismatch DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	120
Detector technology	PIXEL
Collection date	2019-09-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	40.350, 58.433, 101.142
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.240 - 2.020
Rwork	0.219
R-free	0.26340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4e9g
RMSD bond length	0.009
RMSD bond angle	1.085
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.17)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.040
High resolution limit [Å]	2.010	2.010
Rpim	0.031	0.524
Number of reflections	15766	732
<I/σ(I)>	27.9
Completeness [%]	97.7
Redundancy	9.8
CC(1/2)		0.728

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	15% PEG1500 0.1 M MES pH 6.5 0.2 M sodium acetate