6VJH

The crystal structure of the 2009 H1N1/California PA endonuclease wild type in complex with SJ000986192

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	I 4 2 2
Unit cell lengths	88.971, 88.971, 133.396
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.460 - 2.550
R-factor	0.2265
Rwork	0.225
R-free	0.24660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vpt
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.020	2.660
High resolution limit [Å]	2.550	2.550
Rmerge	0.083	0.817
Rmeas	0.093	0.913
Rpim	0.041	0.400
Number of reflections	8461	1001
<I/σ(I)>	9.4	2
Completeness [%]	94.6	95.6
Redundancy	5.1	4.9
CC(1/2)	0.997	0.758

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M HEPES PH 7.8, 1 M AMMONIUM SULFATE, 10 MM MNCL2, 10 MM MGCL2, 0.5% PVP K15