6VFF

Dimer of Human Adenosine Deaminase Acting on dsRNA (ADAR2) mutant E488Q bound to dsRNA sequence derived from human GLI1 gene

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	170.000, 63.210, 142.200
Unit cell angles	90.00, 118.13, 90.00

Refinement procedure

Resolution	37.480 - 2.800
R-factor	0.1991
Rwork	0.197
R-free	0.24830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ed2
RMSD bond length	0.008
RMSD bond angle	1.247
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.480	37.480	2.870
High resolution limit [Å]	2.800	12.520	2.800
Rmerge	0.094	0.019	1.181
Rmeas	0.104	0.021	1.306
Number of reflections	32384	385	2260
<I/σ(I)>	14.14	58.86	1.56
Completeness [%]	97.3	92.8	92.1
Redundancy	5.522	5.244	5.422
CC(1/2)	0.998	1.000	0.611

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	50mM MOPS pH 7.0, 20% w/v PEG 4000, 100 mM NaCl