6VC2
LRH-1 bound to SS-RJW100 and a fragment of the Tif2 Coactivator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2015-03-20 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 46.608, 46.608, 220.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.381 - 1.697 |
R-factor | 0.2105 |
Rwork | 0.210 |
R-free | 0.22840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5l11 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.760 |
High resolution limit [Å] | 1.697 | 3.660 | 1.700 |
Rmerge | 0.110 | 0.115 | 0.609 |
Rmeas | 0.117 | 0.122 | 0.682 |
Rpim | 0.039 | 0.041 | 0.292 |
Number of reflections | 27544 | 3093 | 2314 |
<I/σ(I)> | 14.9 | ||
Completeness [%] | 97.4 | 98.5 | 84.9 |
Redundancy | 6.7 | 8.2 | 3.7 |
CC(1/2) | 0.991 | 0.788 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | Sodium acetate, PEG 4000, glycerol |