6VAH

Crystal structure of human TEAD2 transcription factor in complex with Flufenamic acid derivative

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	120.792, 61.307, 79.862
Unit cell angles	90.00, 118.10, 90.00

Refinement procedure

Resolution	39.070 - 2.110
R-factor	0.2055
Rwork	0.203
R-free	0.24250
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5dq8
RMSD bond length	0.009
RMSD bond angle	1.383
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.093	0.050	0.551
Rmeas	0.105	0.057	0.645
Rpim	0.048	0.027	0.326
Total number of observations	117595
Number of reflections	28671	1467	1282
<I/σ(I)>	9.2
Completeness [%]	97.0	95.1	88.2
Redundancy	4.1	4	2.9
CC(1/2)		0.996	0.847

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	3.5M Na Formate, and 0.1M Bis-Tris Propane pH7.0