6V4R
Crystal structure of a chimeric MR78-like antibody chimera-1 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-11-04 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 133.780, 133.780, 91.910 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 57.930 - 3.480 |
R-factor | 0.2961 |
Rwork | 0.295 |
R-free | 0.32730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jrp |
RMSD bond length | 0.020 |
RMSD bond angle | 2.178 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.930 | 3.540 |
High resolution limit [Å] | 3.480 | 3.480 |
Rmeas | 0.342 | 4.159 |
Rpim | 0.084 | 0.995 |
Total number of observations | 206074 | |
Number of reflections | 12393 | 618 |
<I/σ(I)> | 8.8 | 1.1 |
Completeness [%] | 99.2 | 98.7 |
Redundancy | 16.6 | 17.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287 | 4.5 M NaCl, 0.1 M HEPES, pH 7.5 |