6V3N

Crystal structure of CDYL2 in complex with H3K27me3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792368
Spacegroup name	P 43 2 2
Unit cell lengths	60.792, 60.792, 178.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.650 - 2.700
R-factor	0.241
Rwork	0.240
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hae
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.650	44.650	2.830
High resolution limit [Å]	2.700	8.960	2.700
Rmerge	0.085	0.031	1.097
Rmeas	0.089	0.033	1.138
Number of reflections	9869	336	1256
<I/σ(I)>	26.8	64.8	3
Completeness [%]	99.9
Redundancy	13.6	9.8	14
CC(1/2)	0.999	0.999	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	1.6 M ammonium sulfate, 0.01 M magnesium chloride, 0.1 M sodium cacodylate