6V2H
Crystal structure of CDYL2 in complex with H3tK27me3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-30 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97957 |
| Spacegroup name | H 3 |
| Unit cell lengths | 210.750, 210.750, 67.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 63.140 - 2.600 |
| R-factor | 0.2185 |
| Rwork | 0.217 |
| R-free | 0.23660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | an early version of PDB entry 6V41 (CDY1 in complex with a histone peptide) |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.362 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 63.060 | 63.060 | 2.670 |
| High resolution limit [Å] | 2.600 | 11.630 | 2.600 |
| Rmerge | 0.099 | 0.052 | 0.997 |
| Rmeas | 0.109 | 0.057 | 1.095 |
| Rpim | 0.045 | 0.024 | 0.452 |
| Total number of observations | 200420 | 2090 | 14724 |
| Number of reflections | 34195 | 374 | 2515 |
| <I/σ(I)> | 14.8 | 41.7 | 1.9 |
| Completeness [%] | 100.0 | 98.9 | 100 |
| Redundancy | 5.9 | 5.6 | 5.9 |
| CC(1/2) | 0.997 | 0.996 | 0.658 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 23% PEG3350, 0.25 M ammonium dihydrogen phosphate, 0.1 M sodium cacodylate |
