6V0P
PRMT5 complex bound to covalent PBM inhibitor BRD6711
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 99.110, 138.570, 178.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.730 - 1.880 |
| Rwork | 0.187 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gqb |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.608 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.100 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmeas | 0.115 | 1.600 |
| Number of reflections | 50976 | 1020 |
| <I/σ(I)> | 32.7 | 1.1 |
| Completeness [%] | 93.9 | 86 |
| Redundancy | 6.5 | 5.5 |
| CC(1/2) | 0.998 | 0.480 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 281 | 26% PEG3350 200 mM ammonium sulfate 0.05% w/v DDM |
