6V0N
PRMT5 bound to PBM peptide from Riok1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97857 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 105.960, 139.410, 179.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.750 - 2.110 |
| R-factor | 0.204 |
| Rwork | 0.203 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gqb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.750 | 2.220 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmeas | 0.012 | |
| Number of reflections | 76646 | 1533 |
| <I/σ(I)> | 10.65 | 0.85 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 6.3 | 4.6 |
| CC(1/2) | 0.998 | 0.436 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 281 | 30% PEG 3350 200 mM ammonium sulfate 0.05% w/v dodecyl-maltoside |
