6UZK

Crystal Structure of the Ternary Complex of KRIT1 bound to both the Rap1 GTPase and HKi6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2019-06-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.999995
Spacegroup name	P 1 21 1
Unit cell lengths	57.204, 77.089, 58.410
Unit cell angles	90.00, 91.56, 90.00

Refinement procedure

Resolution	36.514 - 1.924
Rwork	0.220
R-free	0.28610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hdo
RMSD bond length	0.002
RMSD bond angle	1.069
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.920	1.920
Number of reflections	36147	1384
<I/σ(I)>	16.33
Completeness [%]	94.0
Redundancy	3
CC(1/2)	0.991	0.486

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20-25% PEG 3,350, 100 mM Tris, pH 8.5, 100 mM KCl