6UY3
Structure of anti-hCD33 conditional scFv with methotrexate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-12 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0 |
Spacegroup name | I 21 3 |
Unit cell lengths | 118.992, 118.992, 118.992 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.500 - 2.000 |
R-factor | 0.1903 |
Rwork | 0.189 |
R-free | 0.22060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6uup |
RMSD bond length | 0.002 |
RMSD bond angle | 0.515 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.500 | 2.073 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 18953 | 1883 |
<I/σ(I)> | 21.68 | |
Completeness [%] | 99.3 | |
Redundancy | 19 | |
CC(1/2) | 0.999 | 0.645 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.15M Cesium chloride, 15% w/v PEG 3350 |