6UXJ
Structure of serine hydroxymethyltransferase 8 from Glycine max cultivar Essex complexed with PLP-glycine and 5-formyltetrahydrofolate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2018-04-29 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.889, 90.764, 146.850 |
Unit cell angles | 90.00, 90.81, 90.00 |
Refinement procedure
Resolution | 45.382 - 1.400 |
R-factor | 0.1659 |
Rwork | 0.165 |
R-free | 0.19370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6uxh |
Data reduction software | XDS |
Data scaling software | Aimless (0.6.3) |
Phasing software | PHASER |
Refinement software | PHENIX (1.14-3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.950 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.070 | 1.019 |
Rmeas | 0.083 | 1.225 |
Rpim | 0.044 | 0.670 |
Total number of observations | 1533812 | 68936 |
Number of reflections | 440251 | 21837 |
<I/σ(I)> | 10.2 | 1 |
Completeness [%] | 98.5 | 98.9 |
Redundancy | 3.5 | 3.2 |
CC(1/2) | 0.998 | 0.504 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.24 M trimethylamine N-oxide, 0.1 M Tris (pH 8.5), 20% (w/v) PEG MME 2000, 10 mM 5-formyl tetrahydrofolate, 20 mM glycine |